Wang, Guangxian
Sun, Jianfei
Yao, Yao
An, Xingshuang
Zhang, Hui
Chu, Guanglei
Jiang, Shui
Guo, Yemin http://orcid.org/0000-0002-5640-443X
Sun, Xia
Liu, Yuan
Funding for this research was provided by:
National Natural Science Foundation of China (31772068)
Special Project of Independent Innovation of Shandong Province (2018CXGC0214)
Shandong Provincial Natural Science Foundation (ZR2017BC001)
Article History
Received: 19 July 2019
Accepted: 22 September 2019
First Online: 12 November 2019
Compliance with Ethical Standards
:
: This article does not contain any studies with human participants performed by any of the authors.
: Not applicable.
: Guangxian Wang declares that there is no conflict of interest. Jianfei Sun declares that there is no conflict of interest. Yao Yao declares that there is no conflict of interest. Xingshuang An declares that there is no conflict of interest. Hui Zhang declares that there is no conflict of interest. Guanglei Chu declares that there is no conflict of interest. Shui Jiang declares that there is no conflict of interest. Yemin Guo declares that there is no conflict of interest. Xia Sun declares that there is no conflict of interest. Yuan Liu declares that there is no conflict of interest.
: A novel enzyme biosensor was developed to assess meat quality and freshness. Using the conductivity of MXene materials and the catalytic activity of Au@Pt nanoflowers, the enzyme biosensor was assembled and prepared for ultra-sensitive and rapid detection of IMP. The MXene-Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> material had a 2D nanostructure similar to graphene, as well as metal conductivity and good biocompatibility. Used as a carrier for related enzymes, MXene provided a good biological environment and a stable microenvironment, while the bimetallic nanoflowers with a core-shell structure had higher catalytic activity than a single noble metal. The by-product hydrogen peroxide was produced by the double-enzyme catalysis process used, the current signal of the catalytic oxidation of hydrogen peroxide by Au@Pt nanoflowers was monitored at the biosensor as the detection target, and the qualitative and quantitative detection of IMP was achieved.