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Authors
Hromcik, Filip https://orcid.org/0000-0001-9859-3198
Holochova, Pavla https://orcid.org/0000-0003-1122-4125
Bohm, Jan https://orcid.org/0000-0002-9723-9493
Kuzelova Kostakova, Eva https://orcid.org/0000-0002-5758-3206
Pokorny, Zdenek https://orcid.org/0000-0002-7098-3693
Ruzicka, Filip https://orcid.org/0000-0001-5679-0513
Borilova Linhartova, Petra https://orcid.org/0000-0003-0953-3615
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Funding
Funding for this research was provided by:
Masaryk University
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Article History
Received: 29 September 2025
Accepted: 22 December 2025
First Online: 4 January 2026
Declarations
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: The authors declare no competing interests.
: This clinical trial was retrospectively registered at ClinicalTrials.gov with ID NCT07149493 on 02/09/2025.
: This study was originally designed as a method validation investigation to refine subgingival probiotic delivery techniques using a newly developed nanofiber dental floss containing Ligilactobacillus salivarius . At the time of ethics approval and initiation (February 2022), the study was not classified as a clinical trial. However, subsequent interpretation of NIH guidelines suggests that the randomized, blinded, crossover design involving a health-related intervention meets the criteria for interventional clinical research. Accordingly, the investigators now recognize that the study should be registered as a clinical trial. The research team is submitting this retrospective registration to ensure transparency and support future publication. No participant-level outcomes have been modified post hoc, and all data were collected prospectively under full ethical oversight.
