Wen, Hairuo
Lou, Xiaoyan
Qu, Zhe
Qin, Chao
Jiang, Hua
Yang, Ying
Kang, Liqing
Geng, Xingchao
Yu, Lei
Huang, Ying
Funding for this research was provided by:
National Key Research and Development Program (2021YFA1101602)
Chinese Academy of Sciences Strategic Leading Science and Technology Project (XDA16040502)
Article History
Received: 8 August 2022
Accepted: 2 November 2022
First Online: 9 November 2022
Declarations
:
: The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. All animal experimental procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of the National Center for Safety Evaluation of Drugs (NCSED, IACUC approval No: IACUC-2021-053, IACUC-2021-060), in compliance with the IACUC Constitution of NCSED, the Guide for the Care and Use of Laboratory Animals (ExternalRef removed) and AAALAC International’s Position Statement. The animals were anaesthetized with isoflurane before blood sampling or necropsy. Adverse events that could be found in this study including, loss of body weight, roachback, piloerection, hind limb paralysis, which are associated with the xenografting of Raji cells. All study protocols (including the research question, key design features, and analysis plan) were prepared before the study and archived at National Center for Safety Evaluation of Drugs. In compliance with the IACUC Constitution of NCSED, euthanasia with CO<sub>2</sub> inhalation is performed, under the following condition(s) as humane endpoints: (1) more than 20% body weight(s) was(were) lost within 3 days, or (2) the animal(s) could not eat/drink on its(their) own, or (3) other important physiological function(s) was (were) lost. In this study, it was lymphoma xenografted in the mice, and no solid metastasis was observed in all the animals. As demonstrated by the bioluminescence intensity, the maximal intensity (tumor burden) of Raji-Luc was about 1 × 10<sup>10</sup> p/sec/cm<sup>2</sup>/sr, the body masses of these animals were reduced no higher than 25% in 14 days, the animals were able to eat and drink, and no obvious physiological function lost was found. Thus, the maximum tumor burden was not exceeded.
: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.