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Authors
Shen, Yanxi
Ye, Bin
Li, Chuanfei
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Funding
Funding for this research was provided by:
General Project of Chongqing Natural Science Foundation (cstc2021jcyj-msxmX0018)
Special Support for Chongqing Postdoctoral Research Project (2021XM3027)
Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University (kryc-yq-2224)
National Natural Science Foundation of China (82103206)
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Article History
Received: 21 February 2025
Accepted: 5 June 2025
First Online: 17 June 2025
Declarations
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: The present study was approved by the Ethics Committee of The Second Affiliated Hospital of Chongqing Medical University (No. 2021 − 242) and written informed consent was provided by all patients prior to the study start. All procedures were performed in accordance with the ethical standards of the Institutional Review Board and The Declaration of Helsinki, and its later amendments or comparable ethical standards.
: The reason for “physical cropping” of the membrane before primary antibody incubation is that it can improve detection sensitivity, save antibodies and reagents, achieve multi-target detection, avoid signal cross-contamination, and adapt to different experimental needs.
: The authors declare no competing interests.
