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Article History

Accepted: 1 August 2022

First Online: 16 October 2022

Declarations

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: Animals were maintained in the laboratory animal facility of ACTREC following the national guidelines mentioned by the Committee for the Purpose of Control and Supervision of the Experiments on Animals (CPCSEA), Ministry of Fisheries, animal Husbandry and Dairying department of animal Husbandry and Dairying, Government of India. The <i>Institutional Animal Ethics Committee</i> (IAEC) of the Advanced Center for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Center, India, Registration no. 65/GO/ReBiBt/S/99/CPCSEA approved the protocol (IAEC protocol no. 07/2017). The animal studies were performed only after taking prior consent and approval from the Institutional Animal Ethics Committee, ACTREC. All the experiments were conducted in strict accordance for the care and use of the laboratory animals. A controlled environment was provided to the animals with a temperature of 22 ± 2 °C and relative humidity maintained at 40–70%. A 12-h day night cycle was maintained (7:00 to 19:00 day and 19:00 to 7:00 night). The animals were given gamma irradiated balanced diet (Altromin 1314P) and sterile water. Individually ventilated Cage system (IVC, M/S Citizen, India) was used to house mice. These IVCs were provided with autoclaved corn cob as bedding for the mice. Animal euthanasia was done under the supervision of attending veterinarian using CPCSEA recommended euthanizing agent, carbon dioxide.

: Not applicable.

: The authors declare no competing interests.