Liu, Sha
Chen, Daoyuan
Zhu, Xiaosu
Wang, Xiaowen
Li, Xiao
Du, Yuan
Zhang, Peng
Tian, Jingwei
Song, Yingjian
Funding for this research was provided by:
Natural Science Foundation of Shandong Province (ZR2023MH318)
Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China (2022KJ344)
Article History
Accepted: 7 February 2024
First Online: 21 February 2024
Declarations
:
: All institutional and national guidelines for the care and use of laboratory animals were followed. Sprague–Dawley rats and nude mice were provided from the Jinan Pengyue Experimental Animal Breeding co., ltd (Jinan, China). The animal experiments conducted in this study adhered to the UK Animals (Scientific Procedures) Act, 1986, and associated guidelines, as well as the EU Directive 2010/63/EU for animal experiments. The ARRIVE criteria were followed in all animal experiments. During 1 week of acclimation time, the rats and nude mice were housed four per cage in a 12 h light/dark cycle with food and water available ad libitum. The dry powder formulation developed in this study was a local drug delivery preparation, and the tumor micro- environment of lung was complex in vivo. Seventy-four rats and 20 nude mice were necessarily used to explore the in vivo distribution, pharmacokinetic study, antitumor effect, and safety evaluation in this study. All animal experiments were approved by Yantai University Laboratory Animal Ethics Committee, affiliated to Yantai University (approval number, YTDX 20220615).
: All authors read and approved the final manuscript and agree with its publication.
: The authors declare no competing interests.