Sun, Jiaqi
Wang, Xiyang
Shen, Qingsong
Wang, Min
Chen, Shuxian
Zhang, Xuechun
Huang, Yongping
Zhang, Zhonglin
Li, Wenhua
Yuan, Yufeng
Huang, Zan http://orcid.org/0000-0003-2817-6182
Funding for this research was provided by:
National Natural Science Foundation of China (91957109, 81870427)
Article History
Accepted: 17 August 2022
First Online: 3 November 2022
Declarations
:
: The authors have stated explicitly that there are no conflicts of interest in connection with this article.
: HCC tissues and the paired adjacent non-tumor tissues were collected from Zhongnan Hospital of Wuhan University, Wuhan, China. Ethic approval was obtained from the Medical Ethics Committee of Zhongnan Hospital of Wuhan University (No.2018013). All procedures performed in this study adhere to the tenets of the <i>Declaration of Helsinki</i>.All animal experiments were approved by the Animal Care and Use Committee of the College of Life Sciences of Wuhan University (No.16010 A), and care was provided according to the criteria outlined in the <i>Guide for the Care and Use of Laboratory Animals</i> prepared by the National Academy of Sciences and published by the National Institutes of Health.
: Informed consent was obtained from each patient included in this study.
: Not applicable.
: AKT, serine/threonine kinase 1; CCK8, Cell Counting Kit-8; CCL<sub>4</sub>, carbon tetrachloride; CDC20, cell division cycle 20; CDC25C, cell division cycle 25 C; CDK, cyclin dependent kinase; CDK2, cyclin dependent kinase 2; DNA, deoxyribonucleic acid; DNASE1L3, deoxyribonuclease I like 3; DEN, diethylnitrosamine; E2F1, E2F transcription factor 1; gRNA, guide RNA; GSEA, Gene Set Enrichment Analysis ; HCC, hepatocellular carcinoma; HE, hematoxylin-eosin; KEGG, Kyoto Encyclopedia of Genes and Genomes; NET, Neuroendocrine tumor; NRASV12, NRAS proto-oncogene G12V; PCA, Principal Component Analysis; PSR, Picric Sirius red; RNA-Seq, RNA sequencing; RTCA, Real Time Cell Analysis; WT, wild type.