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Article History

Received: 1 November 2020

Revised: 23 November 2020

Accepted: 1 December 2020

First Online: 16 February 2021

Compliance with ethical standards

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: The authors declare no conflicts of interest relevant to this article.

: The study was conducted on crab-eating monkeys (cynomolgus monkey, <i>Macaca fascicularis</i>) in Research Center for Animal Model, Korea Institute of Toxicology (KIT), which is a Good Laboratory Practice facility and located at 30 Baek Hak 1-gil, Jeongeup, Jeollabuk-Do, Republic of Korea. The crab-eating monkeys, which are laboratory non-primate from China (Guangxi Grandforest scientific primate company, Ltd., China), are housed in individual cages (510 W × 800 L × 764 H mm) following 30-day quarantine and acclimation. Size of cage was satisfied the requirements for ‘The Guide for the Care and Use of Laboratory Animals (ILAR publication, 2010 National Academy Press. All were males. The room environment was automatically controlled 20–26 °C, relative humidity 50 ± 10%, 12 h light/12 h dark cycle with 150–300 lx, and ventilation 10 –20 times/hour. Temperature and relative humidity were monitored and recorded daily. Animal room and cage cleaning was performed according to the Research Center for Animal Model’s standard operating procedure. The monkeys were provided food, Lab diet ® #5002, PMI Nutrition International, USA) at 9:00 a.m. and 6:00 p.m. and water ad libitum and were fed approximately 60 g (totally 120 g a day, 60 g twice a day of) of food (Certified Primate Diet #5048, PMI nutrition International, Inc.) twice a day. The animals were managed at KIT, an accredited animal facility, complying with the AAALAC International Animal Care Policies. The Animal Care and Use Committee of the KIT reviewed and approved all the study protocols. All animals were deeply anesthetized by an excess amount of thiopental sodium injection and killed after bleeding. The experimental use of nonhuman primate was performed after receiving approval of the Institutional Animal Care and Use Committee (IACUC) of Korea Institute of Toxicology (IACUC KIT-1811-0441). Information on the liver tissues of each subject is given in Table.