Christopherson, Gregory T.
de Vasconcellos, Jaira F.
Dunn, John C.
Griffin, Daniel W.
Jones, Patrick E.
Nesti, Leon J.
Funding for this research was provided by:
Military Amputee Research Program at Walter Reed Army Medical Center (P05-A011)
Comprehensive Neurosciences Program (CNP-2008-CR01)
Defense Medical Research and Development Program (D10_1_AR_J5_920)
Congressionally Directed Medical Research Programs (W81XWH-13-2-0083)
NIH Intramural Research Program (Z01 AR41131)
USAMRAA BAA (W81XWH-17-2-0009)
Article History
Received: 29 January 2021
Revised: 7 May 2021
Accepted: 13 May 2021
First Online: 7 August 2021
Compliance with ethical standards
:
: The authors declare that they have no competing interests.
: Tissue samples from penetrating traumatic blast extremity injuries sustained by military personal during war conflict were collected approximately 1–2 weeks after injury at the time of surgical debridement at the Walter Reed National Military Medical Center. Uninjured, control muscle samples, were obtained from hamstring harvest of patients undergoing ligamentous knee reconstruction surgery at the Walter Reed National Military Medical Center. The Walter Reed National Military Medical Center Institutional Review Board approved this tissue procurement protocol. The amount of tissue debrided was determined by the operative surgeon. After surgical debridement of the tissue, de-identified samples were placed in a sterile container and transported on ice to the laboratory for processing. All samples were divided for (i) snap freezing in liquid nitrogen, (ii) fixation for scanning electron microscopy evaluation and (iii) isolation of primary mesenchymal progenitor cells (MPCs).
: The contents of this publication are the sole responsibility of the author(s) and do not necessarily reflect the views, opinions or policies of Uniformed Services University of the Health Sciences (USUHS), The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., the Department of Defense (DoD), the Departments of the Army, Navy, or Air Force. Mention of trade names, commercial products, or organizations does not imply endorsement by the U.S. Government.