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A validated FACS-based protocol for the isolation of neuronal and non-neuronal cells from frozen adult brain, suitable for integrated gene and protein analysis

Crossref DOI link: https://doi.org/10.1007/s43440-026-00854-6

Published Online: 2026-04-22

Published Print: 2026-06

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Kolasa, Magdalena https://orcid.org/0000-0002-2023-2699
Roman, Adam https://orcid.org/0000-0003-0197-2453
Mielczarek, Przemysław https://orcid.org/0000-0003-2759-2571
Solich, Joanna https://orcid.org/0000-0002-7818-4263
Faron-Górecka, Agata https://orcid.org/0000-0002-8202-190X
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Text and Data Mining valid from 2026-04-22

Version of Record valid from 2026-04-22
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Article History

Received: 9 January 2026

Revised: 25 March 2026

Accepted: 26 March 2026

First Online: 22 April 2026

Declarations

:

: All animal experiments were carried out in accordance with the Polish Act on the Protection of Animals (Dz.U. z 2020 r. poz. 638), the European Directive 2010/63/EU on the protection of animals used for scientific purposes, and were approved by the II Local Ethics Committee for Animal Experiments. at the Maj Institute of Pharmacology, Polish Academy of Science, Krakow, Poland (permission number: 256/2022).

: The authors declare no competing interests.

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