Lehmann, Johannes http://orcid.org/0000-0002-2833-4411
Narcisi, Roberto http://orcid.org/0000-0003-0521-8618
Franceschini, Natasja
Chatzivasileiou, Danai
Boer, Cindy G.
Koevoet, Wendy J. L. M.
Putavet, Diana
Drabek, Dubravka
van Haperen, Rien
de Keizer, Peter L. J.
van Osch, Gerjo J. V. M. http://orcid.org/0000-0003-1852-6409
ten Berge, Derk http://orcid.org/0000-0002-5863-0860
Funding for this research was provided by:
ZonMw (116006104)
ffg (CartiScaff)
KWF Kankerbestrijding (UMCU7141)
Article History
Received: 23 April 2021
Revised: 18 October 2021
Accepted: 9 November 2021
First Online: 20 January 2022
Declarations
:
: Peter L.J. de Keizer is founder, shareholder and managing director of Cleara Biotech B.V., Utrecht, the Netherlands. Dubravka Drabek and Rien van Haperen are affiliated with Harbour Antibodies, which provided the IL6-neutralizing antibody used in this study. The remaining authors declare no competing financial or other conflicts of interests.
: For collection of human tissue samples, approval was first obtained by the Medical Ethical Committee of the Erasmus MC. Human adult bone marrow-derived MSCs were isolated from femoral bone marrow aspirates of adults undergoing total hip replacement (MEC-2004-142 & MEC-2015-644; age: 43–88, given informed consent). Human paediatric MSCs were derived from left-over iliac crest bone chips of children undergoing palate cleft reconstruction (MEC-2014-16; 9–13 years, by implicit consent). The tissue utilized human tissue was procured as leftover/waste surgical material and it was reviewed and deemed exempt from full ethical review by the Erasmus MC Medical Ethical Committee under code MEC-2014–16. Parents/guardians stated that they did not have any objection to the use of this tissue. Adipose tissue-derived MSCs were isolated from human subcutaneous abdominal adipose obtained as waste material from female donors (age 46–52 years) (MEC-2014–092, by implicit consent). The protocols are in accordance with the ethical standards of our institution and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.