Chetverina, Darya http://orcid.org/0000-0003-0008-3814
Vorobyeva, Nadezhda E.
Mazina, Marina Yu.
Fab, Lika V.
Lomaev, Dmitry
Golovnina, Alexandra
Mogila, Vladic
Georgiev, Pavel
Ziganshin, Rustam H.
Erokhin, Maksim
Funding for this research was provided by:
Russian Science Foundation (18-74-10091)
Article History
Received: 15 November 2021
Revised: 14 April 2022
Accepted: 8 May 2022
First Online: 9 June 2022
Declarations
:
: The authors declare no competing interests.
: Animal handling for the antibody production was carried out strictly according to the procedures outlined in the NIH (USA) Guide for the Care and Use of Laboratory Animals. The protocols used were approved by the Committee on Bioethics of the Institute of Gene Biology, Russian Academy of Sciences. All procedures were performed under the supervision of a licensed veterinarian, under conditions that minimize pain and distress. Rabbits were purchased from a licensed specialized nursery, Manihino. Soviet chinchilla rabbits used in the study are not endangered or protected. Only healthy rabbits, certified by a licensed veterinarian were used. The rabbits were individually housed in standard size, stainless steel rabbit cages, and provided an ad libitum access to alfalfa hay, commercial rabbit food pellets, and water. The appetite and behavior of each rabbit was monitored daily by a licensed veterinarian. Body weight and temperature of each rabbit were evaluated prior to and daily following the immunization. No animals became ill or died at any time prior to the experimental endpoint. At the end of the study period all rabbits were euthanized by intravenous injection of barbiturate anesthetics.
: Not applicable.
: Not applicable.