Bosch, Angela J. T. http://orcid.org/0000-0002-9895-0850
Keller, Lena http://orcid.org/0000-0002-3181-263X
Steiger, Laura
Rohm, Theresa V. http://orcid.org/0000-0001-8498-1018
Wiedemann, Sophia J. http://orcid.org/0000-0002-2632-6952
Low, Andy J. Y.
Stawiski, Marc
Rachid, Leila
Roux, Julien http://orcid.org/0000-0002-4192-5099
Konrad, Daniel http://orcid.org/0000-0001-9067-4356
Wueest, Stephan http://orcid.org/0000-0001-7227-2644
Tugues, Sonia http://orcid.org/0000-0003-1206-3386
Greter, Melanie http://orcid.org/0000-0002-7220-5369
Böni-Schnetzler, Marianne http://orcid.org/0000-0001-5569-5506
Meier, Daniel T. http://orcid.org/0000-0003-3348-4657
Cavelti-Weder, Claudia http://orcid.org/0000-0002-0650-6091
Funding for this research was provided by:
Wolfermann-Naegeli Foundation
Swiss Diabetes Foundation
Philhuman Foundation
Velux Stiftung
Young Independent Investigator Research Grant
University of Basel
Article History
Received: 25 May 2023
Accepted: 7 July 2023
First Online: 4 October 2023
Acknowledgements
: We thank M. Donath (University of Basel, Basel, Switzerland) and his team for their support and expertise and P. Demougin (University of Basel, Basel, Switzerland) for sample preparation. RNA-sequencing was performed at the Genomics Facility Basel of the ETH Zurich. Calculations were performed at the sciCORE (ExternalRef removed) scientific computing centre at the University of Basel (Basel, Switzerland).
: The RNA-Seq dataset is available in the Gene Expression Omnibus (GEO) under the accession number GSE189434 (ExternalRef removed).
: This study was supported by grants from the Velux Foundation, the Philhuman Foundation, the Swiss Diabetes Foundation, the Wolfermann-Naegeli Foundation and the Young Independent Investigator Research Grant (to CC-W). The Swiss National Science Foundation (SNSF) provided salary support to CC-W (32003B_204937 / 2).
: The authors declare that there are no relationships or activities that might bias, or be perceived to bias, their work.
: Experiments were designed by AJTB, SJW, DTM and CC-W. AJTB, LK, LS, TVR, AJYL, DTM and CC-W performed all experiments. SW and DK performed hyperinsulinaemic–euglycaemic clamps and data analysis. MS and LR prepared and stained pancreas section, performed image acquisition and analysed data. SJW designed, performed and analysed the fasting and refeeding experiments and reversal study. IL-1β experiments were designed and executed by AJTB, LS and MB-S. SW and DK contributed technical expertise for performing clamps in mice. MG and ST contributed expertise for AFS98 treatments. Data analysis was performed by AJTB, LK, DTM and CC-W. Analysis was supported by SJW for reversal experiments, MS and LR for beta cell mass quantification and alpha/beta cell ratio, SW and DK for clamps, MG and ST for AFS98 treatment and MB-S and LS for IL1β experiments. JR was involved in the design and analysis of bulk RNA data. AJTB and CC-W wrote the first draft of the manuscript and reviewed and edited the final manuscript. All authors contributed to analysis and discussion of the data. All authors revised and approved the manuscript in its final form. CC-W is the guarantor of this work.