Yu, Vanessa
Yong, Fiona
Marta, Angellica
Khadayate, Sanjay
Osakwe, Adrien
Bhattacharya, Supriyo
Varghese, Sneha S.
Chabosseau, Pauline
Tabibi, Sayed M.
Chen, Keran
Georgiadou, Eleni
Parveen, Nazia
Suleiman, Mara
Stamoulis, Zoe
Marselli, Lorella
De Luca, Carmela
Tesi, Marta
Ostinelli, Giada
Delgadillo-Silva, Luis
Wu, Xiwei
Hatanaka, Yuki
Montoya, Alex
Elliott, James
Patel, Bhavik
Demchenko, Nikita
Whilding, Chad
Hajkova, Petra
Shliaha, Pavel
Kramer, Holger
Ali, Yusuf http://orcid.org/0000-0002-0681-1125
Marchetti, Piero
Sladek, Robert
Dhawan, Sangeeta
Withers, Dominic J.
Rutter, Guy A. http://orcid.org/0000-0001-6360-0343
Millership, Steven J. http://orcid.org/0000-0001-6947-7059
Funding for this research was provided by:
Fonds de Recherche du Québec - Santé
Ministero dell'Istruzione, dell'Università e della Ricerca (PNRR - M4C2-1.3, Project PE_00000019 HEAL ITALIA)
Society for Endocrinology (Summer Studentship, WREC_NCP239, WREC_P93206)
Wellcome Trust (093082/Z/10/Z, 204834/Z/16/Z, WT212625/Z/18/Z)
National Institutes of Health (R01DK120523)
Innovation Canada John R. Evans Leaders Award (CFI 42649)
European Union’s Horizon 2020 research and innovation programme (115881 (RHAPSODY))
Canadian Institutes of Health Research
City of Hope Start-up funds
Wanek Family Foundation to Cure Type 1 Diabetes
California Institute for Regenerative Medicine (EDU4-12772)
Medical Research Council (MC-A654-5QB40, MR/R010676/1, MR/R022259/1)
Division of Diabetes, Endocrinology, and Metabolic Diseases (R01DK135268)
Institute of Nutrition, Metabolism and Diabetes (CIHR-IRSC TDP-186358, JDRF 4-SRA-2023-1182-S-N)
Singapore Ministry of Education Academic Research Fund Tier 2 (MOE-T2EP30221-0003)
Fondation du CHUM (start-up funds)
Article History
Received: 15 November 2023
Accepted: 9 January 2024
First Online: 21 March 2024
Acknowledgements
: We thank the LMS/NIHR Imperial Biomedical Research Centre Flow Cytometry Facility and the MRC LMS Genomics Facility for the support. Some of the data were presented as an abstract at the Society for Endocrinology BES meeting and the 59th EASD Annual Meeting, both in 2023. The graphical abstract and Fig. g were created with BioRender.com.
: RNA-seq data are available from the GEO under accession number GSE249659. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD048465. ImageJ scripts and CellProfiler pipelines can be made available upon reasonable request. Pseudo-time analysis scripts are available via GitHub using the link . Transcriptomic data for <i>ob/ob</i> mice can be obtained from a publicly available source from the Alan Attie laboratory ().
: AMa received a Summer Studentship in the SJM laboratory from the Society for Endocrinology. AO is supported by training scholarships from the Canadian Institutes of Health Research (CIHR) and the Fonds de recherche du Québec – Santé (FRQS). SSV is a CIRM postdoctoral scholar supported by the California Institute for Regenerative Medicine (EDU4-12772). YA is supported by the Singapore Ministry of Education Academic Research Fund Tier 2 (MOE-T2EP30221-0003). PM and MS have been supported by the European Union, Next Generation EU, through the Italian Ministry of University and Research under PNRR - M4C2-1.3, Project PE_00000019 HEAL ITALIA. SD is supported by the National Institutes of Health (NIH) (R01DK120523), by the Wanek Family Foundation to Cure Type 1 Diabetes and by start-up funds from the City of Hope. This work was also supported by a Wellcome Trust Project Grant (093082/Z/10/Z) to DJW and by funding from the Medical Research Council (MRC) to DJW (MC-A654-5QB40). GAR was supported by a Wellcome Trust Investigator award (212625/Z/18/Z), a UKRI-MRC Programme grant (MR/R022259/1), an NIH-NIDDK project grant (R01DK135268), a CIHR-JDRF Team grant (CIHR-IRSC TDP-186358 and JDRF 4-SRA-2023-1182-S-N), CRCHUM start-up funds and an Innovation Canada John R. Evans Leader Award (CFI 42649). GAR and PM received funding from the European Union’s Horizon 2020 research and innovation programme via the Innovative Medicines Initiative 2 Joint Undertaking under grant agreement no. 115881 (RHAPSODY). Work in the SJM laboratory was supported by a Wellcome Trust ISSF Fellowship (204834/Z/16/Z, award no. RSRO_67869) and by a Society for Endocrinology Early Career Grant (WREC_P93206) and Small Equipment Grant (WREC_NCP239).
: PM is a member of the Editorial Board of <i>Diabetologia</i>. GAR has received grant funding from and is a consultant for Sun Pharmaceuticals Inc. The authors declare that there are no other relationships or activities that might bias, or be perceived to bias, their work.
: SJM and GAR designed the research. VY, FY, AMa, SK, AO, SB, SSV, PC, SMT, KC, EG, NP, MS, ZS, LM, CDL, MT, GO, LD-S, XW, YH, AMo, JE, BP, ND, CW, PH, PS, HK, YA, PM, RS, SD, DJW and SJM performed and interpretated experiments and analyses. PH, PM, RS, SD and DJW contributed new reagents/analytical tools. SJM and GAR drafted and/or wrote the manuscript. SJM, SD, DJW and GAR provided funding. SJM and GAR supervised the work. SJM serves as the guarantor of this work. All authors critically reviewed and approved the manuscript according to the ICMJE criteria.