Ahn, Seungkuk
Ardoña, Herdeline Ann M.
Lind, Johan U.
Eweje, Feyisayo
Kim, Sean L.
Gonzalez, Grant M.
Liu, Qihan
Zimmerman, John F.
Pyrgiotakis, Georgios
Zhang, Zhenyuan
Beltran-Huarac, Juan
Carpinone, Paul
Moudgil, Brij M.
Demokritou, Philip
Parker, Kevin Kit
Funding for this research was provided by:
Harvard University Materials Research Science and Engineering Center (NSF Award No. DMR-1420570)
Harvard Center for Nanoscale Systems (CNS), a member of the National Nanotechnology Infrastructure Network (NSF Award No. 1541959)
National Institute of Environmental Health Sciences of the National Institutes of Health as part of the Nanotechnology Health Implications Research (NHIR) Consortium (NIH Grant No. U01ES027272)
Wyss Institute for Biologically Inspired Engineering at Harvard University
Article History
Received: 3 March 2018
Revised: 10 April 2018
Accepted: 23 April 2018
First Online: 10 May 2018
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: The Faculty of Arts and Sciences (FAS) of Harvard University maintains an Institutional Animal Care and Use Committee (IACUC) as required by the Public Health Service (PHS) Policy on Humane Care and Use of Laboratory Animals. All protocols for animal experiments done in this study were approved by IACUC at Harvard University. All animals were appropriately housed prior to use. On arrival to our animal facility, neonatal rat pups used in the study and their dams were housed in clean, ventilated microisolator cages in a climate-controlled, automatic light cycle-equipped room with dam having free access to food and fresh water. All research personnel handling animals were appropriately qualified and trained by Harvard’s Office of Animal Resources under the direction of the Attending Veterinarian. Our euthanasia method for the pups is consistent with the recommendations of the 2013 American Veterinary Medical Association (AVMA) Guidelines on Euthanasia for rodents. All rats used in this study were cared for and used in a manner that complies with the US Government Principles for the Utilization and Care of Vertebrate Animals Used in Testing, Research, and Training [CitationRef removed]; the Guide for the Care and Use of Laboratory Animals [CitationRef removed]; and the Animal Welfare Act/Regulations [CitationRef removed].