Leone, Robert D.
Sun, Im-Meng
Oh, Min-Hee
Sun, Im-Hong
Wen, Jiayu
Englert, Judson
Powell, Jonathan D.
Funding for this research was provided by:
Corvus Pharmaceuticals
Bloomberg Kimmel Institute for Immunotherapy
Article History
Received: 23 January 2018
Accepted: 8 June 2018
First Online: 19 June 2018
Compliance with ethical standards
:
: Jonathan D. Powell has been a paid consultant for Corvus and has equity in the company. All other authors declare that they have no conflicts of interest.
: All applicable international and national guidelines for the care of animals were followed. All mouse procedures approved by Johns Hopkins University Institutional Animal Care and Use Committee (Protocol #M016M103, approved 4/1/2016).
: C57BL/6 obtained from Charles River Laboratories (MC38 experiments) or Jackson Laboratories (ACT; 000664). OT-I and CD90.1, BALB/c mice obtained from The Jackson Laboratory. Male or female mice were used for each experiment; mice were sex and age matched accordingly.
: MC38 cells were donated by CORVUS pharmaceuticals. The identity and specific pathogen free status of these cells was validated by microsatellite genotype analysis (IDEXX Bioresearch). B16-OVA melanoma cells were a gift from Hyam Levitsky. All other tumor cell lines used were obtained from the ATCC. All cell lines were mycoplasma free via ELISA-based assays performed every 6 months.