Alexander, Matthew P. http://orcid.org/0000-0003-4124-2834
Fiering, Steven N. http://orcid.org/0000-0003-0624-974X
Ostroff, Gary R. http://orcid.org/0000-0003-4202-4556
Cramer, Robert A. http://orcid.org/0000-0001-5503-5006
Mullins, David W. http://orcid.org/0000-0002-2644-350X
Funding for this research was provided by:
Joanna M. Nicolay Melanoma Foundation
National Cancer Institute (P30CA023108, R03 CA188418)
National Institute of General Medical Sciences (P30GM103415)
Article History
Received: 5 October 2017
Accepted: 13 August 2018
First Online: 23 August 2018
Compliance with ethical standards
:
: David W. Mullins is a senior scientific advisor and has received research grants, unrelated to the current work, from Qu Biologics (Vancouver, BC). The remaining authors declare no conflict of interest.
: C57BL/6J (stock no. 000664) and CCR2<sup>−/−</sup> mice (stock no. 004999) mice were obtained from Jackson Laboratories (Bar Harbor, ME). CCR2.DTR mice were a gift from Dr. Tobias Hohl (Memorial Sloan Kettering Cancer Center). Myeloid-HIF1α<sup>−/−</sup> mice (LysM-Cre floxed-HIF1α exon 2) were generated and bred in house at Dartmouth by Dr. Robert A. Cramer.
: All animal care procedures and experimental protocols were performed in accordance with all applicable international, national, and/or institutional guidelines for the care and use of animals. All animal care procedures and experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of Dartmouth College (protocol 1141).
: B16-F10 were newly acquired from ATCC, then expanded and cryopreserved to create individual aliquots that were used for each study. B16.F10-luciferase cells were generated from new aliqouts of B16-F10. Cells were confirmed to be free of <i>Mycoplasma</i> infection using the Hek Blue Mycoplasma Detection Kit from Invivogen.