Lin, Lin
Rayman, Patricia
Pavicic, Paul G. Jr.
Tannenbaum, Charles
Hamilton, Thomas
Montero, Alberto
Ko, Jennifer
Gastman, Brian
Finke, James
Ernstoff, Marc
Diaz-Montero, C. Marcela
Funding for this research was provided by:
National Cancer Institute (K01CA134927, R21CA188767)
Velosano Pilot Research Award
Article History
Received: 28 February 2018
Accepted: 28 November 2018
First Online: 14 December 2018
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: All animals were housed under specific pathogen-free conditions in accordance with Institutional and Federal guidelines at the Cleveland Lerner Research Institution and the experiments were approved by the local Institutional Animal Care and Use Committee (IACUC). Human samples were collected under IRB-approved tissue collection protocol number 3164.
: Informed consent was obtained prior to sample collection.
: C57BL/6 (Thy1.1−), Pmel-1 transgenic (Thy1.1+Vβ13+), IFNγR1 knockout mice were purchased from Jackson Laboratory (Bar Harbor, ME).
: B16-F10 cells, derived from a gp100+ spontaneous murine melanoma cell line, were obtained from American Type Culture Collection (ATCC) (Manassas, VA). Cell culture was performed under standardized protocols to ensure that phenotypically similar cells are implanted during each experiment. In our laboratory, cell lines are routinely tested for pathogens, and expanded to produce stock aliquots frozen at the same passage.