Malyshkina, Anna http://orcid.org/0000-0001-5199-5472
Littwitz-Salomon, Elisabeth
Sutter, Kathrin
Ross, Jean Alexander
Paschen, Annette
Windmann, Sonja
Schimmer, Simone
Dittmer, Ulf
Funding for this research was provided by:
Wilhelm Sander-Stiftung (2014.091.1)
Article History
Received: 29 August 2018
Accepted: 6 January 2019
First Online: 11 January 2019
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Animal experiments were performed in strict accordance with the German regulations of the Society for Laboratory Animal Science (GV-SOLAS) and the European Health Law of the Federation of Laboratory Animal Science Associations (FELASA). The protocol was approved by the North Rhine-Westphalia State Agency for Nature, Environment and Consumer Protection (LANUV) (Permit number: G 1518/15). All efforts were made to minimize suffering.
: Female C57BL/6 mice between 6 and 10 weeks old were purchased from Envigo, Germany.
: The FBL-3 cell line is a Friend virus-induced leukemia cell line, generated in a C57BL/6 mouse. The EL-4 cell line is a chemically induced lymphoma cell line, generated in a C57BL/6 mouse by 9,10-dimethyl-1,2-benzanthracene. Both cell lines were a gift from Kim J. Hasenkrug (Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA). They were expanded, aliquoted, and frozen for further use. Once in culture, cells were not continuously passaged. The identity of the cell lines was confirmed by biological assays. Before the experiments, cell lines were tested in naïve C57BL/6 mice: FBL-3 cells inoculated into the right flank of the mice were rejected, whereas EL-4 cells were not.