Han, Zhen
Liu, Shuo
Lin, Hongsheng
Trivett, Anna L.
Hannifin, Sean
Yang, De
Oppenheim, Joost J. http://orcid.org/0000-0003-0271-7921
Article History
Received: 24 July 2018
Accepted: 1 April 2019
First Online: 3 June 2019
Compliance with ethical standards
: All experiments with mice were performed in compliance with the principles and procedures outlined in the National Institutes of Health Guide for the Care and Use of Animals and were approved by the National Cancer Institute at Frederick Animal Care and Use Committee. Animal research approval number: ASP16-294.
: All authors declare that they have no conflict of interest.
: C57BL/6 mice were obtained from Charles River (Frederick, MD). Nude, TLR7<sup>−/−</sup>, and MyD88<sup>−/−</sup> mice were purchased from the Jackson Laboratory (Bar Harbor, ME).
: All cell lines used in the present study were purchased from the American Type Culture Collection (ATCC, Manassas, VA). The cell lines were expanded and cryopreserved according to the culture and cryopreserving conditions recommended by American Type Culture Collection. Both Hepa1-6 hepatoma cell line (CRL-1830) and EG7 thymoma cell line (CRL-2113) were examined by with Molecular Testing of Biological Materials (MTBM) test (Animal Health Diagnostic Laboratory, NCI-Frederick) to verify the lack of microbial contamination. For any experiment involving the use of a given cell line, one cryopreserved vial was used, avoiding additional cell line authentication.