Chen, Yen-Lin
Huang, Wen-Chien
Lin, Feng-Ming
Hsieh, Huangpin B.
Hsieh, Chia-Hsun
Hsieh, Ruey Kuen
Chen, Kuo-Wei
Yen, Ming-Hong
Lee, James
Su, Stephen
Marfatia, Twinkal
Chang, Shih-En
Sundar, Padma
Patterson, Bruce
Watson, Drew
Mei, Rui
Javey, Manana http://orcid.org/0000-0002-1706-0523
Article History
Received: 24 November 2018
Accepted: 3 May 2019
First Online: 14 May 2019
Compliance with ethical standards
:
: The following authors are employees of CellMax Life: Feng-Ming Lin, Huangpin B. Hsieh, Stephen Su, Twinkal Marfatia, Shih-En Chang, Padma Sundar, Drew Watson, Rui Mei, and Manana Javey. The rest of the authors have no conflicts of interest to disclose: Yen-Lin Chen, Wen-Chien Huang, Chia-Hsun Hsieh, Ruey Kuen Hsieh, Kuo-Wei Chen, Ming-Hong Yen, James Lee, Bruce Patterson
: Analysis of the deidentified subjects was performed retrospectively and thus is exempt from IRB approval per the ethical standards of Cardinal Tien Hospital, Mackay Memorial Hospital, Chang Gung Memorial Hospital, Cheng Hsin General Hospital and Cathay General Hospital.
: All study subjects provided written consent. By signing the written informed consent, the patients gave permission for processing their blood and tissue samples for research purposes.
: Eight human tumor cell lines (3 breast cancer lines: T47D, SK-BR3, MDA-MB-468; 3 lung cancer cell lines: H1975, H661, H520; 1 colorectal cancer cell line: HT29; and 1 prostate cancer cell line: LNCaP) were purchased from the American Type Culture Collection (ATCC, USA) and cultured according to ATCC guidelines. Two human tumor cell lines, a colorectal cancer cell line (HCT-116) and a prostate cancer cell line (PC3), were purchased from the Bioresource Collection and Research Center (BCRC, Taiwan) and cultured according to the manufacturer`s instructions. The PD-L1 positive control cell line and the PD-L1 negative control cell line were obtained from InCellDx, Inc. (IncellDx, Menlo Park, CA, USA). All cell lines were cultured and propagated according to the manufacturers’ instructions and phenotyped for relevant protein markers during the downstream procedures.