Langhans, Bettina http://orcid.org/0000-0001-7733-1092
Nischalke, Hans Dieter
Krämer, Benjamin
Dold, Leona
Lutz, Philipp
Mohr, Raphael
Vogt, Annabelle
Toma, Marieta
Eis-Hübinger, Anna Maria
Nattermann, Jacob
Strassburg, Christian P.
Gonzalez-Carmona, Maria Angeles
Spengler, Ulrich
Funding for this research was provided by:
German Center for Infection Research
Article History
Received: 23 November 2018
Accepted: 30 October 2019
First Online: 13 November 2019
Compliance with ethical standards
:
: The authors declare that they have no conflicts of interest.
: The study protocol conformed to the ethical guidelines of the 1975 Declaration of Helsinki and was approved by the Institutional Review Board of the Bonn University Ethics Committee (Approval #150/15).
: All patients and healthy blood donors gave written informed consent prior to blood sample collection for the use of biomaterials and clinical data for scientific purposes.
: The mouse mastocytoma cell line P815 and the various hepatoma cell lines HepG2, Hep3B, and Huh7 were purchased from the American Type Culture Collection (ATCC<sup>®</sup>) TIB64™. Cell lines Huh4 and HepT1 were a kind gift of W. Caselmann and T. Pietsch, respectively. Cell line authentication for human HepG2, Hep3B, Huh7, Huh4, and HepT1 was not necessary, because these cell lines were excluded from our in vitro studies due to up-regulation of PD-L1/L2 in cell culture. Cell line authentication of murine P815 cells was performed by the Swiss DNA company Microsynth (Supplementary Table 3).