Burke, Shannon http://orcid.org/0000-0002-2876-7946
Shergold, Amy
Elder, Matthew J.
Whitworth, Justine
Cheng, Xing
Jin, Hong
Wilkinson, Robert W.
Harper, James
Carroll, Danielle K.
Article History
Received: 23 September 2019
Accepted: 20 January 2020
First Online: 22 February 2020
Compliance with ethical standards
:
: All authors are or were employees of AstraZeneca at the time the research was performed, with stock and/or stock options or interests in AstraZeneca.
: Human bloods samples were obtained from National Health Service Blood and Transplant Service, Cambridge, UK. AstraZeneca obtained a favorable ethics review from the East of England—Cambridge East Research Ethics Committee for the MedImmune Research Tissue Bank, RTB 16/EE/0334. PBMC preparations were from Cellular Technology Limited, (OH, USA) and no approval from any ethics committee was required.
: Human peripheral blood mononuclear cells were isolated from leukocyte preparations supplied by National Health Service Blood and Transplant Service, Cambridge, UK, or Cellular Technology Limited (OH, USA), as anonymized samples from consenting donors.
: HT10180 fibrosarcoma cells and MDA-MB-231 breast cancer cells were purchased from American Type Culture Collection, MEL624 melanoma cells were from Amplimmune. Cell lines have been STR profiled by IDEXX BioAnalytics (MO, USA).