Costanzo-Garvey, Diane L.
Keeley, Tyler
Case, Adam J.
Watson, Gabrielle F.
Alsamraae, Massar
Yu, Yangsheng
Su, Kaihong
Heim, Cortney E.
Kielian, Tammy
Morrissey, Colm
Frieling, Jeremy S.
Cook, Leah M. http://orcid.org/0000-0002-9996-7419
Funding for this research was provided by:
American Cancer Society (PF-13-175-01-CSM, RSG-19-127-01-CSM)
National Institutes of Health (R00HL123471)
National Cancer Institute (P50CA97186)
National Institute of Allergy and Infectious Diseases (P01 AI083211)
Article History
Received: 24 July 2019
Accepted: 17 February 2020
First Online: 29 February 2020
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: Fox Chase SCID Beige mice were obtained from Charles River Laboratory (USA). C57Bl/6 J mice were obtained from Jackson Laboratories (Bar Harbor, ME, USA). Animals were maintained on a 12-h light/dark schedule and had free access to laboratory chow (Teklad LM 485) and water.
: All human cell lines used in this manuscript were processed by Genetica Cell Line Testing (a LabCorp brand; Burlington, NC, USA) for authentication testing using analytical procedures for DNA extraction, polymerase chain reaction (PCR) and capillary electrophoresis on a 3130xl genetic analyzer (Applied Biosystems). The thirteen core CODIS short tandem repeat (STR) loci plus PENTA E and PENTA D, and the gender-determining locus, amelogenin, were analyzed using the commercially available PowerPlex<sup>®</sup> 16HS amplification kit (Promega Corporation; mouse marker included) and GeneMapper ID v3.2.1 software (Applied Biosystems). Appropriate positive and negative controls were used concurrently throughout the analysis. Authentication of each cell line was confirmed by entering the STR DNA profile of each tested cell line into known repository cell line databases (i.e. ATCC, DSMZ); authentication is defined as having a percent match with the reference STR profile at or above 80% when using the ANSI/ATCC guidelines (ASN-0002-2011) OR having a “unique” STR DNA profile (no matches found) for “in-house” cell lines not distributed by any cell line repository.
: Patient prostate cancer samples were obtained from patients who died of metastatic CRPC and who signed written informed consent under the aegis of the Prostate Cancer Donor Program at the University of Washington [CitationRef removed]. The study was approved by the institutional review board at the University of Washington. Human bone marrow was purchased from the Lonza Walkersville, Inc. operates a Research Bone Marrow Donor Program (Walkersville, MD, USA) to recruit, screen, test, evaluate and select donors, who sign informed consent, for collection of bone marrow samples for research use. This Donor Program is currently approved, has been approved for over 10 years, and is submitted for annual approval by a commercial institutional review board. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the University of Nebraska Medical Center. The study was approved by the Institutional Committee for the Use and Care of Laboratory Animals of University of Nebraska Medical Center (Omaha, NE, USA). Animal research approval number: 17-113-12.