Barr, Adele M. http://orcid.org/0000-0003-3989-9570
Silva, Anabel
Prato, Sandro
Belz, Gabrielle T.
Maraskovsky, Eugene
Baz Morelli, Adriana
Funding for this research was provided by:
National Health and Medical Research Council (APP1006663, APP1135898)
Article History
Received: 18 September 2019
Accepted: 27 April 2020
First Online: 9 May 2020
Compliance with ethical standards
:
: Adele Barr, Sandro Prato, Adriana Baz Morelli and Eugene Maraskovsky are currently employees of CSL Ltd. At the time of completing this work Anabel Silva was an employee of CSL Ltd. The authors declare that there are no other conflicts of interest.
: This work was carried out according to the ‘Australian Code for the Responsible Conduct of Research 2018’. All animal studies were approved by the CSL Limited/Pfizer Australia Animal Ethics Committee (Melbourne, VIC, Australia). Experiments performed were outlined in approved animal ethics applications AEC (Animal Ethics Committee) register numbers 863, 831, 880, 966 and 981.
: C57BL/6J and B6.SJL/J-PTPRCa mice used in these experiments were bred at the Bio21 Institute (Melbourne, VIC, Australia). C57BL/6 Tg(TRAMP)8247Ng/J and B6.129S7-<i>Ifng</i><sup><i>tm1Ts</i></sup>/J mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA).
: The B16-OVA melanoma cell line (clone MO5, [CitationRef removed]) was kindly provided by A. Surhbier (QIMR Berghofer, Brisbane, QLD, Australia). The TRAMP-C1 cell line was purchased from American Type Culture Collection (ATCC®) (CRL-2730). The Eµ-myc-GFP-OVA lymphoma cell line was kindly provided by the creator of this lymphoma cell line, J. Villadangos [CitationRef removed] (The University of Melbourne, Melbourne, Australia). Tumor cell lines have been cultured no longer than 3 weeks after thawing and used for the experiments without any further authentication. All cell lines were routinely tested for mycoplasma contamination with PCR technique.
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