Vitale, Laura A.
He, Li-Zhen
Thomas, Lawrence J.
Wasiuk, Anna
O’Neill, Thomas
Widger, Jenifer
Crocker, Andrea
Mills-Chen, Laura
Forsberg, Eric
Weidlick, Jeffrey
Patterson, Colleen
Hammond, Russell A.
Boyer, James
Sisson, Crystal
Alvarado, Diego
Goldstein, Joel
Marsh, Henry C. Jr.
Keler, Tibor http://orcid.org/0000-0003-1757-4230
Funding for this research was provided by:
Celldex Therapeutics
Article History
Received: 3 March 2020
Accepted: 13 May 2020
First Online: 25 May 2020
Compliance with ethical standards
:
: All authors are employees of, and own stock or stock options in Celldex Therapeutics, Inc.
: Animals were sourced from IACUC-approved sources. Human CD27 transgenic mice studies (animal source: Celldex) and murine xenograft studies (animal source: Taconic Biosciences) were approved by the Celldex IACUC of Hampton, NJ (AUP CDX-002, AUP CDX-003). Animal care followed the Guide for the Care and Use of Laboratory Animals, Eighth Edition (National Research Council 2011, Washington, DC, The National Academic Press). The pilot primate study (animal source: Covance) was approved by the Citoxlab IACUC of Stilwell, KS (AUP 2118-3843). Animals were handled according to The Canadian Council of Animal Care and AAALAC rules (Association for Assessment and Accreditation of Laboratory Animal Care International). Cell lines were sourced directly from vendors that provide authentication. HD-BIOP3 cells were sourced from Horizon Discovery. HEK-293 cells transfected with human CD27 or PD-L1 were sourced from Crown Bioscience. HEK-293 NFκB-luciferase reporter cell line was sourced from Signosis, Inc. Reporter cells for the PD-1 NFAT and ADCC assays were sourced from Promega. The Raji, Ramos and MDA-MB-231 cell lines were sourced from ATCC. BCL1 cells were sourced from Dr. M.J. Glennie, Southampton University. Human whole blood and leukapheresis were sourced commercially from Biological Specialty Company, Colmar, PA. Human biologicals are collected at their FDA-registered and IRB-approved donor centers.
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