Di Napoli, Arianna http://orcid.org/0000-0002-3159-5380
Greco, Daniele
Scafetta, Giorgia
Ascenzi, Francesca
Gulino, Alessandro
Aurisicchio, Luigi
Santanelli Di Pompeo, Fabio
Bonifacino, Adriana
Giarnieri, Enrico
Morgan, John
Mancini, Rita
Kadin, Marshall E.
Funding for this research was provided by:
Sapienza Università di Roma (Ateneo 2017)
Associazione Italiana per la Ricerca sul Cancro (IG17009)
LazioInnova (85-2017-13750)
Ministero dell’Istruzione, dell’Università e della Ricerca (PRIN 2017)
Aesthetic Surgery Education and Research Foundation (8580)
Università degli Studi di Roma La Sapienza
Article History
Received: 23 July 2020
Accepted: 20 October 2020
First Online: 4 November 2020
Compliance with ethical standards
:
: The authors declare no potential conflicts of interest.
: The study was performed in accordance with the Declaration of Helsinki ethical guidelines for medical research involving human subjects and approved by the Ethics Committee of Sant’Andrea Hospital/University “Sapienza” of Rome (EC n. 82 SA_2017) and Roger Williams Medical Center IRB (#18-522-99). The Animal Welfare Committee of the University of Rome Sapienza (Rome) in accordance with the European Guidelines for Animal Care and Use of Laboratory Animals approved all experiments with rodents (Project Authorization # A69A0.2.EXT.12). All the procedures have been performed in accordance with “Directive 2010/63/EU on the protection of Animals used for scientific purposes” and made effective in Italy by the Legislative Decree DLGS 26/2014.
: Written informed consent was obtained from patients upon sample collection. Patients consented to the use of their specimens and data for research and publication.
: Six-week-old female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice (NSG) obtained from Charles River were used in this work.
: Primary tumor cells derived from seromas of three patients diagnosed with BI-ALCL at Sant’Andrea Hospital, Roma, Italy were used in this work. Two out of 3 primary cultured cells were successfully authenticated using Short Tandem Repeat (STR) analysis by ATCC (n. STRA11550 and STRA11552). TLBR-1,-2,-3,-4 are cell lines derived from clinical cases of BIA-ALCL and were kindly provided by Dr. Alan L. Epstein, MD PhD (Keck School of Medicine, University of Southern California). Mac-1, Mac-2A are cutaneous T cell lymphoma lines established by MEK and, Karpas 299 are an ALK-positive systemic ALCL line obtained from the DSMZ German Collection of Microorganisms and cell cultures GmbH. No cell line authentication was necessary.