García-Escudero, Vega
Ruiz-Gabarre, Daniel
Gargini, Ricardo
Pérez, Mar
García, Esther
Cuadros, Raquel
Hernández, Ivó H.
Cabrera, Jorge R.
García-Escudero, Ramón
Lucas, José J.
Hernández, Félix
Ávila, Jesús http://orcid.org/0000-0002-6288-0571
Funding for this research was provided by:
Ministerio de Ciencia, Innovación y Universidades (PGC2018-096177-B-00)
Centro de Investigación Biomédica en Red Enfermedades Neurodegenerativas
Fundación Científica Asociación Española Contra el Cáncer
Article History
Received: 27 October 2020
Revised: 21 April 2021
Accepted: 22 April 2021
First Online: 2 May 2021
Declarations
:
: The authors declare that they have no competing interests to disclose.
: All applicable international, national, and institutional animal welfare guidelines were followed for the experiments involving experimentation animals. All procedures were in accordance with ethical standards and were approved by the pertinent Ethics Committee. Mice were bred in the animal facility of Centro de Biología Molecular Severo Ochoa. They were housed in a specific pathogen-free colony facility under standard laboratory conditions, following European Community Guidelines (directive 86/609/EEC), and handled in accordance with European and local animal care protocols (PROEX 62/14 and 291/15). 4–5 mice were housed per cage with food and water available ad libitum, and maintained in a temperature-controlled environment on a 12 h/12 h light/dark cycle with light onset at 8 a.m.
: Human brain samples were obtained from Banco de Tejidos (Fundación CIEN, Instituto de Salud Carlos III, Madrid, Spain). Written informed consent for tissue donation for research purposes was obtained for all individuals following their approval procedures.