Lima, Karol Sartori
Salles, Antônio Eduardo Sparça
de Araújo Costa, Gabriel
Yokoyama, Márjori Frítola http://orcid.org/0000-0001-9187-5870
de Paula Ramos, Solange
Paixão-Côrtes, Vanessa Rodrigues
de Lima, Renata Lúcia Leite Ferreira
Salles, Maria José Sparça
Article History
Received: 22 April 2020
Accepted: 17 August 2020
First Online: 31 August 2020
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: All procedures described herein comply with ARRIVE guidelines and are following the National Institutes of Health (NIH) guidelines regarding the care and use of animals for experimental procedures (National Research Council, 2011). They were also approved by the Ethics Committee for Animal Experimentation of the State University of Londrina (9397.2014.90) and carried out with the consent of each author.
: To guarantee the welfare of the animals from the sample of this study, the research team considered some practices during the experimental phase: the maintenance of controlled conditions of light and temperature in the bioterium; unlimited access to water and feed; the allocation of animals in the proportion of two female mice for each male mice, in cages, measuring 30 × 20 cm × 13 cm, made of polypropylene with a zinc-plated wire cover and lined with wood shavings; the realization of daily treatments by only two people out of the research team, most quickly and comfortably; euthanasia most quickly and comfortably.