dos Santos, Tiago Marcon http://orcid.org/0000-0002-7328-6092
Siebert, Cassiana http://orcid.org/0000-0002-3002-472X
Bobermin, Larissa Daniele http://orcid.org/0000-0001-8318-4818
Quincozes-Santos, André http://orcid.org/0000-0001-8611-4890
Wyse, Angela T. S. http://orcid.org/0000-0001-8769-1147
Funding for this research was provided by:
Conselho Nacional de Desenvolvimento Científico e Tecnológico (Edital Universal (Proc. 401507/2016), INCT (EN 465671/2014-4))
Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (PRONEX (16/2551-0000465-0))
Article History
Received: 7 October 2020
Accepted: 18 July 2021
First Online: 29 July 2021
Declarations
:
: The authors declare no conflict of interest. All authors have approved the final article.
: Wistar male rats (thirty days old, 70–80 g) were obtained from the Central Animal House of Biochemistry Department, Institute of Basic Health Sciences at the Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. Animals were maintained with lights on 7:00 a.m. at 7:00 p.m. (12:12 h light/dark cycle) in a colony room constant temperature (22 ± 1 °C), with ad libitum 20% protein commercial chow (w/w) and water (3–4 animals per home cage). The rats were randomized into two groups: control and HCY. Every effort was made to minimize the number of animals and the distress caused throughout the experiment. Official government guidelines for animal care by the Brazilian Federal Law No. 11,794 of October 08, 2008, the NIH Guide to the Care and Use of Laboratory Animals (NIH Publication No. 80–23, 1996), and the ARRIVE guidelines were followed to work development. The experimental protocol was approved by UFRGS Ethics Committee for Animals Use (#33301/2017). The reagents and part of the inputs used in the assays were purchased from Sigma-Aldrich (San Luis, Missouri, EUA) or Merck Millipore (Burlington, Massachusetts, EUA).