Funding for this research was provided by:
European Maritime and Fisheries Fund (PFEA470016FA1000002 (NutriEgg))
Article History
Received: 15 January 2020
Accepted: 22 June 2020
First Online: 30 June 2020
Compliance with ethical standards
: Experimentations were conducted in the INRAE PEIMA experimental facility (Sizun, France—Agreement number B29-277-02). All fish were reared and handled in strict accordance with French and European policies and guidelines of the INRAE PEIMA Institutional Animal Care and Use Ethical Committee, which specifically approved this study. Fish were monitored daily during the experiment. If any clinical symptoms (i.e., morphological abnormality, restlessness, or uncoordinated movements) were observed, fish were sedated by immersion in MS-222 solution at a concentration of 50 mg L<sup>−1</sup> and then euthanized by immersion in a MS-222 solution at a concentration of 400 mg L<sup>−1</sup> (anesthetic overdose) in 3 min.