Wolin, Ingrid A. V.
Nascimento, Ana Paula M.
Seeger, Rodrigo
Poluceno, Gabriela G.
Zanotto-Filho, Alfeu
Nedel, Claudia B.
Tasca, Carla I.
Correia, Sarah Elizabeth Gomes
Oliveira, Messias Vital
Pinto-Junior, Vanir Reis
Osterne, Vinicius Jose Silva
Nascimento, Kyria Santiago
Cavada, Benildo Sousa
Leal, Rodrigo Bainy http://orcid.org/0000-0003-2536-6888
Funding for this research was provided by:
Conselho Nacional de Desenvolvimento Científico e Tecnológico (#309048/2019-2, 439601/2018-5, RENORBIO 407945/2013-0)
Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (CP 26/2020 #2021TR000310, FAPESC/PRONEX Program - NENASC #1262/2012-9)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Finance Code 001)
Article History
Received: 11 March 2022
Revised: 18 November 2022
Accepted: 30 November 2022
First Online: 16 December 2022
Declarations
:
: The authors have declared that there are no conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.
: This study was performed in compliance with the Helsinki Declaration on ethical principles for handling human tissue specimens, with all Brazilian national regulations and requirements. Glioblastoma Multiforme (GBM1) cells were surgically isolated from a GBM tumor from a patient at Hospital Celso Ramos in Florianopolis, Santa Catarina, Brazil and cultured at Santa Catarina Federal University (UFSC/Brazil). All procedures, and patient consent forms were approved by the UFSC/Brazil, Human Research Ethics Committee (CEPSH protocol number 108.286). Primary astrocyte cultures were prepared from cerebral cortex male newborn Wistar rats. The animals were provided by the Animal Care Facility at the Federal University of Santa Catarina (UFSC). All procedures were approved by the Institutional Animal Care and Use Committee (CEUA/UFSC protocol number 2380161216). Rat C6 glioma cells (ATCC-CCL-107) and human U87 glioma cells (ATCC/ HTB-14) lineages were provided by the Cell Bank of Rio de Janeiro (RJ, Brazil).