Funding for this research was provided by:
Brazilian National Council for Scientific and Technological Development (#402626/2012 5, #402364/2012 0, #382692/2011)
State of Rio Grande do Sul Foundation for Research Support (# 002106 2551/13 5)
Text and Data Mining valid from 2016-08-13
Received: 23 May 2016
Accepted: 6 August 2016
First Online: 13 August 2016
Compliance with ethical standards
: CMS, TGH, LPOJ, and PIHBJ designed the study. CMS, CVM, GSS, and TGH completed all the experiments described in this manuscript. CMS, LPOJ, TGH, and PIHBJ analyzed the results. CMS and PIHBJ co-wrote the article. PIHBJ provided experimental advice and revised the final version of the manuscript. All the authors had final approval of the submitted and published versions.
: The authors declare no conflict of interest and no competing interests such as consultancies, financial involvement, patent ownership, etc. in relation to the work described herein. CNPq, FAPERGS and CAPES (the funding organisms) had no involvement in the propositions presented in this manuscript.
: All the procedures performed in studies involving the animals followed the ethical rules established by Arouca’s Act (Federal Law 11794/2008) and the Guide for Care and Use of Experimental Animals published by the National Institutes of Health (NIH publication no. 85-23, revised in 1996). The procedures were approved by the Federal University of Rio Grande do Sul Ethics Committee on Animal Experimentation (CEUA #23451), according to the guidelines of the Brazilian National Council for the Control of Animal Experimentation (CONCEA).