Mousavifard, Arezoo
Pourazizi Najafabadi, Elahe
Akhavan Rahnama, Mahshid
Anbarlou, Azadeh
Atashi, Amir
Funding for this research was provided by:
Islamic azad university najafabad branch
Article History
Received: 22 February 2020
Accepted: 9 May 2020
First Online: 11 June 2020
Ethical approval and consent to participate
: This experimental study was approved by the Ethical Committee of Islamic Azad University (No. 135896). Adipose tissue of abdomen was obtained from donors undergoing liposuction with the informed consent. Harvested tissues were placed immediately in phosphate-buffered Saline (PBS) (Invitrogen) containing 1% penicillin/streptomycin (Invitrogen). Adipose-derived MSCs were isolated by collagenase digestion method (collagenase type I, Invitrogen). Then, the cells were re-suspended in Dulbecco’s Modified Eagle’s Medium (DMEM, Bio-Idea, Iran) supplemented with 10% Fetal Bovine Serum (FBS) (Invitrogen) and 1% penicillin/streptomycin (Invitrogen) and incubated at 37°C.