Article History
Accepted: 28 March 2021
First Online: 4 April 2021
Declarations
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: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The animal studies were performed after receiving approval of the Animal Care and Use Committee of the Faculty of Pharmaceutical Sciences (ethical approval number: FP/PT/019/A/010). Clinically normal male albino Wistar rats weighing 200 ± 10 g, procured from the animal breeding center, Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka and housed in a regulated environment (25 ± 2 °C, 12 h light/dark cycle, light period starting at 7 am), acclimatized for 1 week prior to study and provided free access to standard rodent pellets (Guinea feeds Ltd Nigeria) and water in clean glass water bottles ad libitum, were used for the experiment. All animal experiments complied with the ARRIVE guidelines and were performed in accordance with the U.K. Animals (Scientific Procedures) Act, 1986 and associated guidelines, EU Directive 2010/63/EU for animal experiments. Cage-side clinical observations of the rats were made throughout the study period. The rats were divided into nine groups of five rats each and fasted overnight prior to the induction of diabetes mellitus.
: The authors declare no competing interests.