Questel, Jennifer M. http://orcid.org/0000-0003-0169-6102
Hopcroft, Russell R.
DeHart, Hayley M.
Smoot, Caitlin A.
Kosobokova, Ksenia N.
Bucklin, Ann
Funding for this research was provided by:
NOAA Research (NA15OAR0110209)
Article History
Received: 5 December 2019
Revised: 14 September 2020
Accepted: 29 October 2020
First Online: 9 January 2021
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed by the authors.
: All necessary permits for sampling and observational field studies have been obtained by the authors from the competent authorities and are mentioned in the acknowledgments, if applicable.
: The V4 and V9 nuclear 18 rRNA and mitochondrial COI metabarcoding data generated and analyzed during this study have been deposited in the NCBI GenBank Short Read Archive (ExternalRef removed). Raw sequence reads in FASTQ format from the NOAA Hidden Ocean 2016: Chukchi Borderland expedition can be accessed using SRA BioProject ID PRJNA593255. Eukaryotic 18S rRNA sequences were deposited in GenBank under the accession numbers MG660871-MG661071 and MN784560-MN784617. Metazoan mitochondrial COI sequences were assigned GenBank accession numbers MN831487-MN831677. Sequences for 18S and COI used to complete species representation in the ArCop databases were mined from the NCBI GenBank public repository. Bioinformatic scripts, taxonomic mapping, and corresponding FASTA sequence files formatted for Mothur for each DNA marker are available at ExternalRef removed.
: J.Q. conducted laboratory methods for metabarcoding, bioinformatics, created the ArCop databases, analyzed data, and wrote the manuscript. C.S., J.Q., K.K., and R.H. identified specimens used in the ArCop database. C.S., J.Q., and R.H. collected zooplankton samples used for metabarcoding analysis. H.D. sequenced 18S and COI for the ArCop databases. R.H. and A.B. acquired funding and designed the research. A.B. provided molecular ecology expertise. All authors contributed to writing the manuscript.