Arisandi, Dewi Puspa
Paradisa, Fragaria Vesca
Sugiharto, Bambang
Avivi, Sholeh
Fanata, Wahyu Indra Duwi http://orcid.org/0000-0002-4992-9799
Funding for this research was provided by:
Ministry of Research, Technology and Higher Education of the Republic of Indonesia. (0551/UN25.3.1/LT/2017)
Article History
Accepted: 28 April 2020
First Online: 12 May 2020
Compliance with ethical standards
:
: The authors declare that they have no competing interest.
: Pendok, Genjah Arum, and Merah are aromatic rice varieties originated from East Java, Indonesia. We subjected these three rice varieties to anther culture optimization using culture media supplemented with either of ethylene inhibitor, auxin, and sugar. Among the three varieties, we found that Pendok showed the highest rate in callus formation and plant regeneration, whereas Merah Wangi showed the lack of response to those two parameters. Furthermore, maltose is the best sugar for callus induction in Pendok, and 2,4-D is the suitable auxin for callus induction. Finally, we found that ethylene inhibitor was only effective for callus induction rate of recalcitrant-type Merah Wangi.