Diao, Ningning
Liu, Yan
Wang, Wenxin
Cao, Min
Liu, Xiaowei
Yang, Weili
Cao, Yuxin
Sun, Tianying
Pei, Huijie
Guo, Chunjing
Chen, Daquan http://orcid.org/0000-0002-6796-0204
Funding for this research was provided by:
Natural Science Foundation of Shandong Province (No. ZR2019ZD24, ZR2019YQ30)
Graduate Innovation Foundation of Yantai university (GIFYTU)
Taishan Scholar Foundation of Shandong Province (No. qnts20161035)
Article History
Accepted: 16 March 2024
First Online: 31 March 2024
Declarations
:
: All experiments were conducted by the ARRIVE Guidelines and were approved by the Animal Ethics Committee of Yantai University (Ethical Annotation License Number: YTU20220923). Male Sprague-Dawley rats (180 ∼ 200 g) were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd. (Shandong, China). All animals have free access to food and water and are housed at a temperature of 22 ± 1℃, relative humidity of 50 ± 1%, and a light/dark cycle of 12/12 h. Before administration, the rats were anesthetized with intra-peritoneal injection of anesthetics (80 mg/kg ketamine and 4.5 mg/kg xylazine) while monitoring body temperature with a feedback-conditioned heating pad. Rats were euthanized by intraperitoneal injection of excessive pentobarbital sodium. The study does not involve human subjects.
: All authors have read and agreed to the published version of the manuscript.
: The authors declare no competing interests.