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Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

Crossref DOI link: https://doi.org/10.1038/nbt.3437

Published Online: 2016-01-18

Published Print: 2016-02

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Doench, John G

Fusi, Nicolo https://orcid.org/0000-0002-4102-0169
Sullender, Meagan https://orcid.org/0000-0001-6666-8650
Hegde, Mudra

Vaimberg, Emma W

Donovan, Katherine F

Smith, Ian

Tothova, Zuzana

Wilen, Craig

Orchard, Robert

Virgin, Herbert W

Listgarten, Jennifer https://orcid.org/0000-0002-6600-1431
Root, David E
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Text and Data Mining valid from 2016-01-18
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Article History

Received: 7 May 2015

Accepted: 19 November 2015

First Online: 18 January 2016

Competing interests

: N.F. and J.L. are employed by Microsoft Research.