Kataura, Tetsushi http://orcid.org/0000-0002-3958-5232
Sedlackova, Lucia http://orcid.org/0000-0003-4482-088X
Sun, Congxin
Kocak, Gamze http://orcid.org/0000-0003-1164-367X
Wilson, Niall
Banks, Peter
Hayat, Faisal
Trushin, Sergey
Trushina, Eugenia http://orcid.org/0000-0002-6291-2860
Maddocks, Oliver D. K. http://orcid.org/0000-0002-5551-9091
Oblong, John E.
Miwa, Satomi
Imoto, Masaya
Saiki, Shinji
Erskine, Daniel
Migaud, Marie E. http://orcid.org/0000-0002-9626-2405
Sarkar, Sovan http://orcid.org/0000-0002-9456-4362
Korolchuk, Viktor I. http://orcid.org/0000-0002-4071-592X
Funding for this research was provided by:
Uehara Memorial Foundation
MEXT | Japan Society for the Promotion of Science (19J12969, 18KK0242, 18KK0242, 18KK0242)
Fellowships from the International Medical Research Foundation and Newcastle University
Fellowship from TUBITAK
Mitchell Cancer Institute funding
U.S. Department of Health & Human Services | National Institutes of Health (RF1AG55549)
Cancer Research UK (C53309/A19702)
Alzheimer’s Research UK (ARUK-SRF2022A-006)
Wellcome Trust (109626/Z/15/Z, 1516ISSFFEL10)
LifeArc (P2019-0004, Pathfinder 2324602)
UK-India Education and Research Initiative (2016-17-0087)
University of Birmingham
RCUK | Biotechnology and Biological Sciences Research Council (BB/M023389/1, BB/R008167/2)
RCUK | MRC | Medical Research Foundation (BH174490)
Article History
Received: 16 November 2023
Revised: 19 May 2024
Accepted: 22 May 2024
First Online: 31 May 2024
Competing interests
: J.E.O. is an employee of The Procter & Gamble Company, USA. S. Sarkar is a scientific consultant for Ayin Tech LLC. V.I.K. is a Scientific Advisor for Longaevus Technologies.
: The study was performed in accordance with the Declaration of Helsinki. The control and NPC1 patient-derived fibroblasts were obtained from Coriell Cell Repositories and therefore exempt from institutional committee approval. The control and NPC1 patient-derived iPSCs were originally generated in the lab of Rudolf Jaenisch at the Whitehead Institute for Biomedical Research using protocols approved by the relevant Institutional Review Board (Massachusetts Institute of Technology) and the Embryonic Stem Cell Research Oversight Committees (Whitehead Institute). These iPSC lines, generated from human fibroblasts from Coriell Cell Repositories and have been previously published, were used for this study in the lab of Sovan Sarkar at the University of Birmingham under material transfer agreement UBMTA 15-0594. All experiments were performed in accordance with ISSCR and institutional guidelines and regulations at the University of Birmingham.