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Cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide CRISPR insertions without template donor

Crossref DOI link: https://doi.org/10.1038/s41421-019-0120-z

Published Online: 2019-10-01

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Shi, Xin

Shou, Jia

Mehryar, Mohammadreza M.

Li, Jingwei

Wang, Leyang

Zhang, Mo

Huang, Haiyan

Sun, Xiaofang

Wu, Qiang
Funding

Funding for this research was provided by:

National Natural Science Foundation of China (91640118)

National Natural Science Foundation of China (31470820)

National Natural Science Foundation of China (31630039)

National Natural Science Foundation of China (91640118)

National Natural Science Foundation of China (31470820)

Ministry of Science and Technology of the People's Republic of China (2017YFA0504203)

Ministry of Science and Technology of the People's Republic of China (2018YFC1004504)

Ministry of Science and Technology of the People's Republic of China (2017YFA0504203)

Ministry of Science and Technology of the People's Republic of China (2018YFC1004504)
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Article History

Received: 14 March 2019

Accepted: 6 August 2019

First Online: 1 October 2019

Conflict of interest

: The authors declare that they have no conflict of interest.

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