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Article History

Received: 12 October 2023

Revised: 11 April 2024

Accepted: 19 April 2024

First Online: 22 May 2024

Competing interests

: The authors declare no competing interests.

: The national cohort of human metastatic melanoma patients (Auria cohort) was used in this study following the Declaration of Helsinki. Samples were ethically approved and informed consent from patients was given for biobank research. This approval was granted by the Scientific Steering Ethical Committee of Auria Biobank, as per Decision AB22-4803 on 27.4.2022. The use of these samples aligns with the guidelines set by the Finnish Biobank Act (688/2012), ensuring adherence to the highest ethical research standards. Buffy coats from healthy, anonymous donors were acquired from the Finnish Red Cross (Suomen Punainen Risti Veripalvelu) (license code 16/2022). All animal experiments were performed according to the University of Turku Central Animal Laboratory guidelines, following the Finnish Act on Animal Experimentation (62/2006) in compliance with the 3 Rs principles, in the Central Animal Laboratory (CAL), a certified GLP facility. The license was granted by the local Ethical Committee for Animal Experimentation (ESAVI/39072/2021). All illustrations and representative schematics presented in this manuscript were created using BioRender Suite. The respective license numbers are as follows: Fig. —License DH269QCGFS, Fig. —License NE2690NYCR, Fig. —License VY26L0U4D2, Fig. —License TP269QD4H3, and Supplementary Fig. —License GB26L0Z1RI.