Frosi, Yuri
Lin, Yen-Chu
Shimin, Jiang
Ramlan, Siti Radhiah
Hew, Kelly
Engman, Alf Henrik
Pillai, Anil
Yeung, Kit
Cheng, Yue Xiang
Cornvik, Tobias
Nordlund, Par http://orcid.org/0000-0002-7794-702X
Goh, Megan http://orcid.org/0000-0001-7260-0601
Lama, Dilraj
Gates, Zachary P. http://orcid.org/0000-0003-0588-6157
Verma, Chandra S.
Thean, Dawn
Lane, David P.
Asial, Ignacio
Brown, Christopher J.
Funding for this research was provided by:
Supported by the Agency for Science, Technology and Research (A*STAR).
Article History
Received: 9 March 2021
Accepted: 2 August 2022
First Online: 18 August 2022
Competing interests
: P.N., I.A., K.H. and T.C. are shareholders of DotBio Pte. Ltd. I.A. K.H., A.H.E., A.P., K.Y and Y.X.C., are employees of DotBio Pte. Ltd. The stabilizing mutations introduced to the VH domains described in this paper, as well as the phage display libraries created, are the subject of the following patent applications: WO2016072938A1 (World Intellectual Property Organization, Patent Cooperation Treaty), US20170320934A1 (US Patent and Trademark Office, granted), US11053302B2 (US Patent and Trademark Office, granted), JP2018500879A (Japan Patent Office, granted), EP3215537A4 (European Patent Office, granted), EP3215537B1(European Patent Office, granted), EP4008729A1 (European Patent Office, pending) and CN107001477A (China National Intellectual Property Administration, pending). T.C., I.A. and P.N. are inventors in these patent applications, while Nanyang Technological University is the assignee. These patents are licensed exclusively to DotBio Pte. Ltd. Y.F., Y.C.L., J.S., S.R.R., M.G., D.L., Z.G., C.S.V., D.P.L., D.T. and C.J.B. have no competing interests to declare.