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Authors
Braun, Asolina http://orcid.org/0000-0002-2646-5362
Rowntree, Louise C. http://orcid.org/0000-0002-0649-5089
Huang, Ziyi
Pandey, Kirti
Thuesen, Nikolas
Li, Chen http://orcid.org/0000-0002-1847-754X
Petersen, Jan
Littler, Dene R. http://orcid.org/0000-0001-5904-1905
Raji, Shabana http://orcid.org/0009-0006-1499-6664
Nguyen, Thi H. O. http://orcid.org/0000-0002-9294-7693
Jappe Lange, Emma
Persson, Gry http://orcid.org/0000-0003-2901-7335
Schantz Klausen, Michael
Kringelum, Jens http://orcid.org/0000-0002-7415-3283
Chung, Shanzou
Croft, Nathan P. http://orcid.org/0000-0002-2128-5127
Faridi, Pouya http://orcid.org/0000-0002-2712-3356
Ayala, Rochelle
Rossjohn, Jamie http://orcid.org/0000-0002-2020-7522
Illing, Patricia T. http://orcid.org/0000-0002-3870-0796
Scull, Katherine E. http://orcid.org/0000-0003-4042-7685
Ramarathinam, Sri
Mifsud, Nicole A. http://orcid.org/0000-0001-8647-2102
Kedzierska, Katherine http://orcid.org/0000-0001-6141-335X
Sørensen, Anders Bundgård
Purcell, Anthony W. http://orcid.org/0000-0003-0532-8331
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Article History
Received: 5 November 2023
Accepted: 21 August 2024
First Online: 30 August 2024
Competing interests
: NT, EJL, GP, MSK, JVK, and ABS are employed by Evaxion Biotech A/S which holds IP for identifying neoepitopes and personalized immunotherapy. AWP is on the scientific advisory board of Evaxion Biotech A/S. AWP is on the advisory board of Bioinformatics Solutions (Canada), and Grey Wolf Therapeutics (UK) and is a co-founder of Resseptor Therapeutics (Australia). The remaining authors declare no competing interests.
: Human blood samples were collected between 12-223 days post-SARS-CoV-2 infection in heparinised tubes. Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient centrifugation (Ficoll-Paque, Cytiva) and cryopreserved in fetal calf serum with 10% DMSO (Cat# D2650, Sigma-Aldrich). All human experimental work was conducted according to the Declaration of Helsinki principles and the Australian NHMRC Code of Practice. All blood donors provided written informed consent. Ethics approval was granted from the Human Research Ethics Committee of Melbourne Health (HREC/66341/MH-2020) and The University of Melbourne (13344 and 20782).
