Zhang, Wenkang https://orcid.org/0009-0008-9343-3804
Jiang, Hengfeng https://orcid.org/0009-0006-3895-0935
Han, Liang
Liu, Jie
Wang, Jie https://orcid.org/0000-0001-7602-5050
He, Feng https://orcid.org/0000-0002-8596-1366
Tian, Leilei https://orcid.org/0000-0001-6695-4614
Article History
Received: 29 July 2024
Accepted: 26 February 2025
First Online: 7 March 2025
Competing interests
: This work is related to a pending patent (2024105528370). The patent applicant is the institution Southern University of Science and Technology. LL. T., WK. Z., and F. H. are named as inventors. Status of the application: The patent application was filed at the China National Intellectual Property Administration. The specific aspect of the manuscript covered in the patent application is the protocols of the POSA method and its application in multiplexed protein imaging in cells and tissues. The remaining authors declare no competing interests.
: The HeLa cell line (purchased from Wuhan Servicebio Technology Co., Ltd. China, Cat # STCC10603P) used in this study is a commercially available standard cell line, which does not involve human genetic resources or individual privacy information. No animal or human experiments were included in this study.