White, Laura K. https://orcid.org/0000-0003-2909-6627
Radakovic, Aleksandar
Sajek, Marcin P. https://orcid.org/0000-0002-4115-4191
Dobson, Kezia
Riemondy, Kent A.
del Pozo, Samantha https://orcid.org/0009-0006-4339-6975
Szostak, Jack W. https://orcid.org/0000-0003-4131-1203
Hesselberth, Jay R. https://orcid.org/0000-0002-6299-179X
Funding for this research was provided by:
U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (R35 GM119550)
National Science Foundation (2330283)
Howard Hughes Medical Institute
Article History
Received: 26 February 2025
Accepted: 24 July 2025
First Online: 20 August 2025
Competing interests
: A.R., L.K.W., J.W.S., and J.R.H. have submitted a patent application to the USPTO (PCT/US2025/031145) pertaining to the chemistry, sequencing method, and machine learning aspects of this work. L.K.W. has received travel and accommodation expenses from Oxford Nanopore Technologies to present at scientific meetings, and has participated in beta tests of ONT’s direct RNA sequencing products. L.K.W. is a co-founder of an early-stage entity that may seek to license and commercialize aspects of the technology described in this work. The remaining authors declare no competing interests.