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Authors
Ganguly, Chhandosee https://orcid.org/0009-0008-5135-5318
Aribam, Swarmistha Devi https://orcid.org/0009-0005-3200-5732
dos Santos, Alberto Monteiro https://orcid.org/0000-0002-7033-3922
Martin, Lindsie
Thomas, Leonard M. https://orcid.org/0000-0003-3920-0619
Shao, Yihan
Rajan, Rakhi https://orcid.org/0000-0002-8719-4412
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Funding
Funding for this research was provided by:
National Science Foundation (MCB-1716423 and MCB-2424888)
U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (R35GM153297 and R44GM133270)
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Article History
Received: 30 December 2024
Accepted: 9 January 2026
First Online: 28 January 2026
Competing interests
: R.R. is an inventor on US patents related to FnoCas12a bridge helix variants (US11459552B2 and US12163166B2; status = granted) that were filed by the University of Oklahoma. These patents describe how amino acid substitutions in the bridge helix of Cas12a increase DNA cleavage selectivity. This manuscript presents the structure and mechanism of one of the bridge helix variants (FnoCas12a-K969P/D970P, abbreviated as FnoCas12a KD2P ) that was described in the patents. All other authors declare no competing interests.
