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CRISPR-Cas9 trans-cleavage is hindered by a flanked R-loop, an elongated spacer, and an inactive HNH domain

Crossref DOI link: https://doi.org/10.1038/s41467-026-68789-3

Published Online: 2026-01-24

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Montagud-Martínez, Roser https://orcid.org/0000-0003-4487-1599
Ruiz, Raúl https://orcid.org/0000-0002-5300-2240
Baldanta, Sara https://orcid.org/0000-0002-4558-9774
Delicado-Mateo, Rubén https://orcid.org/0009-0002-7007-7401
Rodrigo, Guillermo https://orcid.org/0000-0002-1871-9617
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Text and Data Mining valid from 2026-01-24

Version of Record valid from 2026-02-25
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Article History

Received: 28 July 2025

Accepted: 16 January 2026

First Online: 24 January 2026

Competing interests

: The authors declare no competing interests.