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Efficient genome editing with chimeric oligonucleotide-directed editing

Crossref DOI link: https://doi.org/10.1038/s41467-026-71624-4

Published Online: 2026-04-13

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Nguyen, Long T.

Rakestraw, Noah R.

Pizzano, Brianna L. M. https://orcid.org/0000-0003-2401-5648
Iyyappan, Rajan https://orcid.org/0000-0002-6356-7093
Young, Cullen B. https://orcid.org/0000-0002-6283-0531
Huang, Yujia

Beerensson, Kate T. https://orcid.org/0009-0002-0517-6868
Fang, Anne

Antal, Sydney G.

Anamisis, Katerina V.

Peggs, Coleen M. D. https://orcid.org/0009-0009-4808-1966
Yan, Jun https://orcid.org/0009-0005-9139-3966
Jing, Yangwode https://orcid.org/0000-0002-1692-6567
Lewis, Jordan G.

Burdine, Rebecca D. https://orcid.org/0000-0001-6620-5015
Adamson, Britt https://orcid.org/0000-0002-9451-5819
Jiang, Zongliang https://orcid.org/0000-0002-7473-3210
Toettcher, Jared E. https://orcid.org/0000-0002-1546-4030
Myhrvold, Cameron https://orcid.org/0000-0002-8971-184X
Jain, Piyush K. https://orcid.org/0000-0001-7153-442X
Funding

Funding for this research was provided by:

U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (R35GM147788)

U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (T32GM136583)

U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (T32GM148739)

U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (R61AI181016)

Funder: New Jersey Commission on cancer Research (NJCCR); Grant Reference Number: COCR24PRG007

U.S. Department of Health & Human Services | NIH | National Human Genome Research Institute (RM1HG009490)

U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD102533)

U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD113698)

U.S. Department of Health & Human Services | National Institutes of Health (U01DK127429)

U.S. Department of Health & Human Services | Centers for Disease Control and Prevention (75D30122C15113)
License Information

Text and Data Mining valid from 2026-04-13

Accepted Manuscript valid from 2026-04-13
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Article History

Received: 9 September 2024

Accepted: 23 March 2026

First Online: 13 April 2026

Competing interests

: L.N., N.R., B.P., C.M. and P.K.J. have filed patent applications with the USPTO/PCT (WIPO) related to chimeric oligonucleotide-directed genome editing and Cas-polymerase-based programmable genome editing technologies described in this work (PCT/US/2025/036584- pending, published; 63/668,340- converted; PCT/US2024/044167- pending, published; 63/600,216- converted; 63/579,160- converted). P.K.J. is a co-founder of CasNx, LLC and CRISPR, LLC. C.M. is a co-founder of Carver Biosciences. B.A. is/was an advisory board member with financial interest in Arbor Biotechnologies and Tessera Therapeutics. J.Y., Y.J., and B.A. are listed as inventors on patent application(s) related to prime editing technologies. J.E.T. is a scientific advisor for Prolific Machines and Nereid Therapeutics. The remaining authors declare no competing interests.

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