Khan, Selina http://orcid.org/0000-0001-7081-1114
Salisch, Nadine C.
Gil, Ana Izquierdo http://orcid.org/0000-0001-9557-1807
Boedhoe, Satish
Boer, Karin Feddes-de
Serroyen, Jan
Schuitemaker, Hanneke http://orcid.org/0000-0001-8563-2266
Zahn, Roland C. http://orcid.org/0000-0003-2822-6231
Funding for this research was provided by:
Janssen Vaccines & Prevention, Leiden, Netherlands
Janssen Vaccines & Prevention, Leiden, Netherlands
Janssen Vaccines & Prevention, Leiden, Netherlands
Janssen Vaccines & Prevention, Leiden, Netherlands
Janssen Vaccines & Prevention, Leiden, Netherlands
Janssen Vaccines & Prevention, Leiden, Netherlands
Janssen Vaccines & Prevention, Leiden, Netherlands
Article History
Received: 9 June 2022
Accepted: 21 October 2022
First Online: 15 November 2022
Competing interests
: The authors declare the following competing interests: all authors are or were employees of Janssen Vaccines & Prevention B.V. while engaged in the research project. S.K., N.C.S., S.B., K.F.B., J.S., and R.C.Z. held or still hold stock in Johnson & Johnson.
: The 3 studies were conducted in facilities assured by the National Institutes of Health Office of Animal Welfare and accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AALAC). The first study was performed at Advanced BioScience Laboratories Inc. (Rockville, MD, USA; purchased by BIOQUAL Inc., Rockville, MD, USA; BIOQUAL: Accreditation # 000624; US Department of Agriculture [USDA] license #51-R-0036). The second study was performed at Charles River Laboratories Edinburgh Ltd (East Lothian, UK; OLAW Foreign assurance: F16-00018 [A5020-01] and Establishment License PEL PCD 60/8607). The third study was performed at Charles River Laboratories, Inc. (Reno, NV, USA; AALAC accredited since 1997 (000918); Office of Laboratory Animal Welfare (OLAW) Animal Welfare Assurance #A4112-01USDA; Research Registration 14-R-0144). All animal research protocols were approved by the Institutional Animal Care and Use Committees at each center, and the studies were conducted in compliance with the Animal Welfare Act, Public Health Service Policy on Humane Care and Use of Laboratory Animals, and other federal statutes and regulations relating to animals and experiments involving animals. Import and export permits for vectors and NHP biospecimens were obtained in compliance with US federal regulations and in accordance with the Convention on International Trade in Endangered Species of Wild Fauna and Flora, as overseen by the US Fish and Wildlife Service.